Whole blood direct PCR method combined with HLA PCR−SSP typing

Direct PCR from Whole Blood

Researchers often purify DNA from blood samples prior to performing PCR because it is believed that blood constituents and the reagents commonly used to preserve blood samples (e.g., anticoagulants) interfere with PCR. In order to save the time and expense required for template purification, several methods have been reported for direct PCR of blood samples. These include microwave irradiation,...

PCR-based HLA class II typing.

T h e h u m a n leukocyte ant igen (HLA) class II molecules are cell-surface glycoproteins involved in the regulat ion of i m m u n e responses. Allelic variation at the HLA class lI loci is an important consideration in major areas of medicine and biology. For instance, detailed knowledge of the allelic constitution at class lI loci is required for match ing of suitable donors and recipients f...

Direct PCR from whole blood, without DNA extraction.

Typically DNA used in PCR assays is usually extracted according to die phenol-chloroform method (1) or by an alternative 'saltingout' rapid purification (2). Moreover partially purified DNA obtained with rapid procedures have been reported to be suitable for PCR amplification (3). We describe here a more simple and efficient method to amplify DNA directly from whole blood samples without any pu...

Alkaline polyethylene glycol-based method for direct PCR from bacteria, eukaryotic tissue samples, and whole blood.

hla-dr typing by pcr-ssp and comparison of serologic results with this method

introduction: considering the role of hla matching in transplant outcome, the quality of hla-dr typing is clearly an important issue.in recent years serologic methods have been replaced with dna based typing methods.the main objective of this study was to compare hla-dr typing data obtained from existing serologic method with that obtained by the new pcr-ssp method. material and methods: fifty-...